HTLV-I Detection and Quantitation Kit

Advantages of HTLV RQ Kit

Ready to Use

Both of the HTLV-I Mix and Albumin Mix contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results for HTLV-I can be viewed in Green/FAM channel

Internal Control

Kit also contains a second set of PCR mix and standards for Albumin as External Control and normalizer. Results can be viewed in Yellow/VIC channel and provides sample cellular load to normalize HTLV-I titer. This Control also prevents false negative results due to extraction failure, PCR inhibition or setup errors

Quality Control

HTLV-Albumin standards & mixes are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR machine and no need to go through time taking manual setting, also reducing setup errors

Kit Specifications

Analytical Sensitivity

2copies/µl for HTLV-I and 1 copy/µl for Albumin

Linear Range

 1million to 10 copies/µl for the cloned target

Reaction Type

Quantitative Real-Time PCR

Detection Method

TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains HTLV-I Mix and Albumin Mix, five standards, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl