Resp II RQ Kit

Advantages of Resp II RQ Kit

Ready to Use

The Resp II Mix contains all necessary reagents for RT-PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results are viewed in Green/FAM, Yellow/VIC, Orange/ROX and Red/Cy5 channels

Internal Control 

Kit also contains reagents for detection of RNase P as an Internal Control which is detected in Orange/ROX channel. Internal Control prevents false negative results due to the extraction failure, reaction inhibition or setup errors

Quality Control

Controls & mixes of Resp II RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors

Kit Specifications

Analytical sensitivity

 13copies/μl for SARS-CoV-2, 10 copies/μl for Influenza A and B, 9 copies/μl for RSV, 8 copies/μl for HPIV-1, 8.4 copies/μl for HPIV-2. 13.6 copies/μl for HPIV-3 and 9.2 copies/μl for HPIV-4.

Reaction type

 Qualitative Real-Time PCR

Detection Method

TaqMan probe, Multiplex (FAM, VIC, ROX, Cy5)

Kit Contents

Kit contains Resp A Mix, Resp B Mix and Resp A positive control, Resp B positive control and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl volume