BCR-ABL (p210) Detection and Quantitation Kit

Detection and Quantitation of BCR-ABL p210 Transcripts (Philadelphia Chromosome)

BCR-ABL also known as Philadelphia chromosome is an abnormality resulted from 9;22 translocation. Consequently, ABL proto-oncogene on chromosome 9 is fused with BCR gene on chromosome 22 (b2a2 or b3a2). This fusion produces mostly 210 or 190 kDa BCR-ABL protein with constitutively active tyrosine kinase activity promoting cell proliferation and inhibition of apoptosis. This fusion gene transcript is detectable in about 95% of CML patients and some cases of ALL. Also, serial monitoring of patients for identifying and measuring BCR-ABL transcripts provides more precise assessment of response to specific therapies and prediction of those in higher risk of disease progression.
BCR-ABL (p210) RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of BCR-ABL transcripts (p210, b2a2 or b3a2 break points only) as well as calculation of BCR-ABL%.

Advantages of MBCR p210 RQ Kit

Ready to Use

The MBCR and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for BCR-ABL and Yellow/VIC channel for ABL.

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Standards & mixes of MBCR RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity

4copies/μl or 0.08% for BCR-ABL in the context of 5,000 copies/μl of ABL transcripts

Reaction Type

Quantitative Real-Time PCR

Detection Method

TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix and 5 quantitation standards for BCR-ABL, PCR Mix and 4 quantitation standards for ABL, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl