HLA-B51/5 Detection Kit

Detection of HLA-B51/5

Human Leukocyte Antigen (HLA-B51/5) is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6. HLA-B51/5 has been associated with Behcet’s disease (BD). Studies show 50%-80% of patients with BD have HLA-B51/5. As a result, tests for HLA-B51/5 are valuable in the diagnosis and control of this disease

HLA-B51/5 RQ Kit provides a ready-to-use Real-Time PCR system for detection of HLA-B51/5

Advantages of HLA-B51/5 RQ Kit

Ready to Use

HLA-B51/5 Mix contain all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels

Internal Control

To examine DNA extraction quality as well as the presence of PCR inhibitors and to prevent false negative results, primers and probe for an Internal Control (a housekeeping gene) is included in the PCR Master Mix and any false negative result due to above errors is prevented

Quality Control

Mix & controls of HLA-B51/5 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument (RotorGene and StepOne)

Kit Specifications

Reaction Type

Qualitative Real-Time PCR

Detection Method

TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix, positive control, negative control, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl

reaction 24reaction ,48reaction ,96reaction	24reaction, 48reaction, 96reaction

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HLA-B51/5 RQ Detection Kit

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