ETV6-RUNX1 Detection and Quantitation Kit

ETV6-RUNX1 Detection and Quantitation Kit

Detection and Quantitation of ETV6-RUNX1 (TEL-AML1) Transcripts

ETV6-RUNX1 (TEL-AML1) is an abnormality resulted from 21;12 translocation. RUNX1 gene, encodes a protein involved in transcriptional control of hematopoiesis. However, as a result of this translocation, it is repressed by ETV6-RUNX1 fusion protein
This alteration occurs in approximately 25% of childhood ALL diagnosed between the ages of 2-10 years
ETV6-RUNX1 (TEL-AML1) RQ provides a ready-to-use Real-Time PCR assay for detection and quantitation of ETV6-RUNX1 transcripts as well as calculation of ETV6-RUNX1 percentage

Advantages of ETV6-RUNX1 RQ Kit

Ready to Use

The ETV6-RUNX1 and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for ETV6-RUNX1 and Yellow/VIC channel for ABL

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results

Quality Control

Standards & mixes of ETV6-RUNX1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors

Kit Specifications

Analytical Sensitivity:  2 copies/μl or 0.02% for TEL-AML1, in context of 10,000 copies/μl of ABL transcripts

Reaction Type

 Quantitative Real-Time PCR

Detection Method

 TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains ETV6-RXN1 Mix and ABL Mix and 5 quantitation standards for ETV6-RUNX1, and 4 quantitation standards for ABL, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl