Factor II Prothrombin Gene Mutation Detection Kit (RQ)

Factor II Prothrombin Gene
Mutation Detection Kit (RQ)

Detection of Factor II (G20210A) Mutation

Prothrombin or factor II is the precursor for thrombin in the coagulation cascade. G20210A mutation in prothrombin gene is the second most prevalent genetic risk factor for venous thromboembolism (VTE) after Factor V Leiden. This mutation increases the risk of venous thrombosis about 2 to 7 times. It has also been linked to increased risk of pregnancy loss. Prevalence of this mutation ranges between 0.5% to 5% depending on the race and ethnic background
The diagnosis of factor II G20210A mutation requires DNA analysis of F2, the gene encoding factor II

Factor II RQ kit provides a ready-to-use Real-Time PCR assay for detection of Factor II (G20210A) mutation

Advantages of F2 RQ Kit 

Ready to Use

The F2 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed in scatter graph analysis or other methods

Internal Control

Due to dual function of the PCR Mix, the main reaction ensures quality of patient sample and DNA extraction and prevents false negative results by PCR inhibition or setup errors

Quality Control

Mix & controls of Factor II RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision

 Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors

Kit Specifications

Differentiation

 WW, WM and MM Genotypes

Reaction Type

 Real-Time PCR

Detection Method

 TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains F2 Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl